The eyes are undoubtedly the most sensitive and delicate organ we possess, and perhaps the most amazing. We rely on our eyesight more than any other sense. Such a human eye faces numerous problems, one such problem with maximum difficulty is dichromacy and achromatopsia, i.e., complete colour blindness. Colour blind is a condition in which people may have mild to severe difficulty in identifying colours. They may not be able to recognise various shades of colours and, in some cases, cannot recognise colours at all. The retinal cone cells are responsible for colour vision. The three basic types of colour blindness are red, green and blue colour blindness-generally called as dichromacy. Red and green colour blindness is more common whereas blue colour blindness-rare type and people cannot distinguish blue or yellow and both the colours are seen as white or grey. Total colour blindness is called achromatopsia. This software proposes a colour vision test using LabVIEW for the early detection of colour blindness and helps to prevent achromatopsia. This virtual instrument is ideal for mass screening in education institutions, clinics, etc., for the early detection of colour blindness.