Three different natural dyes, such as Terminalia kattapa (Red, fruit), Azadirachia indica (Green, leaf), and Clitoria ternatea (Blue, flower), were extracted to fabricate natural dye-sensitized solar cell (N-DSSC) with standard TiO2 nanoparticles (p25) on the FTO base layer. A comparative study was carried out on the performance of RA, GA, and BA-based DSSCs based on optical and electrical properties. The anatase and rutile phases on bare TiO2 film have been confirmed along with the formation of titanium sub-oxides due to the annealing process confirmed by X-ray diffraction. Significant absorption in the visible region has been attained in photo-anodes, which was confirmed with an optical band gap reported from UV reflectance spectra. For the proper electron injection process, a specific functional group has been identified in extracted sensitizer using Fourier transform infrared spectra to ensure the adsorption capability of dye molecules toward TiO2 particles. Photocatalytic properties of photo-anode have been analyzed using photoluminescence (PL) spectra by investigating the rate of recombination via PL intensity. J-V characteristics of fabricated N-DSSC with extracted natural dyes show that the N-DSSC with GA photo-anode offered the highest efficiency (0.79%) among other dyes used in this investigation, such as RA (0.51%) and BA-based photo-anode (0.29%). DSSCs fabricated using photo-anodes RA, GA, and BA (using water as solvent) show excellent outstanding stability and decent efficiency than the ethanol-based cells.