Herein, we report cellulose-based threads from Indian sacred Lotus (Nelumbo nucifera) of the Nymphaceae family embellished with MoS2nanosheets for its enhanced hydrophobic and antimicrobial properties. MoS2nanosheets synthesized by a coprecipitation method using sodium molybdate dihydrate (Na2MoO4·2H2O) and thioacetamide (CH3CSNH2) were used as a sourse for MoS2particle growth with cellulose threads extracted from lotus peduncles. The size, crystallinity, and morphology of pure and MoS2-coated fibers were studied using X-ray diffractometry (XRD) and scanning electron microscopy (SEM). the XRD pattern of pure lotus threads showed a semicrystalline nature, and the threads@MoS2composite showed more crystallinity than the pure threads. SEM depicts that pure lotus threads possess a smooth surface, and the MoS2nanosheets growth can be easily identified on the threads@MoS2. Further, the presence of MoS2nanosheets on threads was confirmed with EDX elemental analysis. Antimicrobial studies with Escherichia coli and Candida albicans reveal that threads@MoS2have better resistance than its counterpart, i.e., pure threads. MoS2sheets play a predominant role in restricting the wicking capability of the pure threads due to their enhanced hydrophobic property. The water absorbency assay denotes the absorption rate of threads@MoS2to 80%, and threads@MoS2shows no penetration for the observed 60 min, thus confirming its wicking restriction. The contact angle for threads@MoS2is 128°, indicating its improved hydrophobicity.