Neurotransmitters serve as critical regulators of nervous system physiology and are essential targets in neurochemical research, necessitating efficient detection and monitoring for the diagnosis and early prevention of neurological disorders. Conventional electrochemical biosensors suffer from limited selectivity, suboptimal electron transfer, and poor stability, impeding their effectiveness in clinical and analytical contexts. To address these limitations, advanced 2D nanomaterials such as MXenes have emerged as prominent sensor interfaces owing to their high conductivity and rich surface chemistry. Hence, the aim of this study was to synthesize and evaluate a novel 2D vanadium carbide (ml-V2CTx) MXene-based sensor for ultrasensitive and selective detection of epinephrine. Electrochemical analysis using differential pulse voltammetry (DPV) revealed an exceptionally low detection limit of 0.2 nM, with a broad linear range spanning 0.05 μM to 250 μM. The sensor demonstrated excellent selectivity against catechol, ascorbic acid, L-tryptophan, acetaminophen, hydroquinone, and 4-nitrophenol. Furthermore, the ml-V2CTx exhibited better repeatability and reproducibility, validating its potential as a reliable sensing platform. This work represents the first report on the use of vanadium-based MXene for electrochemical epinephrine detection, underscoring its promise as a novel material for neurotransmitter monitoring