The present invention provides a novel process route for the production of environmentally friendly, cost competitive, mesoporous bio-calcium silicate from renewable bio-sources and sustainable agricultural by¬products to substitute materials from synthetic sources to utilize them for high performance applications. For the preparation of bio-based calcium silicate, the precursors namely calcium chloride and sodium silicate were derived from calcium rich precursors (viz., egg-shell and sea-shell) and silica rich sources (rice-husk and Mexican grass) respectively through appropriate thermal and chemical experimental methods. The bio-based calcium silicate samples are obtained by gradual addition of sodium silicate to calcium chloride with efficient agitation. The precipitate obtained was washed several times with de-ionized water and dried, and characterized. The samples of different calcium silicate obtained were transformed in to mesoporous bio-calcium silicate at different pH conditions (pH = 2, 4, 6 and 12) using conc.HCi and caustic soda with nonylphenol ether template. The different samples of mesoporous calcium silicate obtained were washed, dried and characterized. The percentage yield of the mesoporous bio-calcium silicate samples obtained from different sources and varying pH (2, 4, 6, 12 ) conditions are in the ranged between 80% and 85%. It was observed that among the samples of meso-porous bio-calcium silicate obtained from different sources, the meso-porous bio-calcium silicate ( PH =2) developed using rice husk ash and egg shell source possesses the highest surface area of 102 g/m2 and the lowest particle size < 50 nm.