LiNiVO₄, a spinel oxide with redox-active metal centres, is known for its chemical stability and multifunctionality. In this study, the LiNiVO₄ based electrode shows a high electrochemically active surface area (Cdl = 1.26 mF cm−2), indicating abundant active sites and efficient ion accessibility. It demonstrates bifunctional electrocatalytic activity with overpotentials of 339 mV and 140 mV at 10 mA cm−2 for OER and HER, with corresponding Tafel slopes of 65 and 284 mV dec−1, conforming to favourable redox kinetics. Furthermore, the antibacterial and cell viability study showed no antibacterial inhibition and high cell viability (98.17% and 69.10% at 4 mg and 8 mg), which is attributed to its chemically stable and non-toxic surface. These results highlight LiNiVO₄ as a promising material for sustainable energy and bio-interface applications.